Speaker: Maria Paola Paronetto
Gene Regulation Program, Center for Genomic Regulation (CRG), Barcelona, Spain

Thursday, 16 September 2010, 16:00h, Aula Fèlix Serratosa

 ABSTRACT

 The Ewing Sarcoma protein EWS belongs to the FET family (FUS, EWS, TAF15) of proteins, which can bind RNA as well as DNA and are implicated in transcription, splicing, RNA transport, signalling and maintenance of genomic integrity. To identify potential alternative splicing events regulated by EWS, RNA from cells depleted of EWS or the corresponding controls were hybridized to a splicing sensitive microarray developed in our laboratory, which analyzes genes relevant for cancer progression and RNA processing. Using this platform, alternative splicing changes were identified that affect key genes involved in the response to DNA damage. Remarkably, some of these changes were also induced upon UV irradiation of control cells. This observation suggests the possibility that changes in EWS activity are part of the mechanisms underlying the changes in alternative splicing induced by genotoxic stress.

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